diff --git a/packages/main/src/components/AnalyticalTable/hooks/useGroupBy.ts b/packages/main/src/components/AnalyticalTable/hooks/useGroupBy.ts
index f6b86fd02bc..cb5187670b5 100644
--- a/packages/main/src/components/AnalyticalTable/hooks/useGroupBy.ts
+++ b/packages/main/src/components/AnalyticalTable/hooks/useGroupBy.ts
@@ -24,6 +24,7 @@ actions.toggleGroupBy = 'toggleGroupBy';
  *
  * This is a fork of react-table's `useGroupBy` with the following changes:
  * - Aggregate grouped columns that define an `aggregate` above their own grouping level, instead of copying the first leaf value
+ * - Derive `canGroupBy` from `disableGroupBy` only (no self-seed), so runtime `groupable` toggling works — like `useSortBy`/`useFilters`
  */
 export const useGroupBy: PluginHook = (hooks: ReactTableHooks) => {
   hooks.getGroupByToggleProps = [defaultGetGroupByToggleProps];
@@ -154,12 +155,11 @@ function useInstance(instance: TableInstance) {
 
     column.canGroupBy = accessor
       ? getFirstDefined(
-          column.canGroupBy,
           columnDisableGroupBy === true ? false : undefined,
           disableGroupBy === true ? false : undefined,
           true,
         )
-      : getFirstDefined(column.canGroupBy, defaultColumnGroupBy, defaultCanGroupBy, false);
+      : getFirstDefined(defaultColumnGroupBy, defaultCanGroupBy, false);
 
     if (column.canGroupBy) {
       column.toggleGroupBy = () => instance.toggleGroupBy(column.id);
diff --git a/packages/main/src/components/AnalyticalTable/test/AnalyticalTableGrouping.gallery.tsx b/packages/main/src/components/AnalyticalTable/test/AnalyticalTableGrouping.gallery.tsx
index 9b1fce5ae65..bf80a9cd252 100644
--- a/packages/main/src/components/AnalyticalTable/test/AnalyticalTableGrouping.gallery.tsx
+++ b/packages/main/src/components/AnalyticalTable/test/AnalyticalTableGrouping.gallery.tsx
@@ -1,4 +1,4 @@
-import { useMemo } from 'react';
+import { useMemo, useState } from 'react';
 import type { AnalyticalTableColumnDefinition } from '../index.js';
 import { AnalyticalTable } from '../index.js';
 
@@ -88,3 +88,32 @@ export const GroupingAggregationHarness = () => {
     />
   );
 };
+
+const toggleData = [
+  { name: 'Alpha', status: 'active' },
+  { name: 'Bravo', status: 'active' },
+  { name: 'Charlie', status: 'inactive' },
+  { name: 'Delta', status: 'active' },
+  { name: 'Echo', status: 'inactive' },
+];
+
+// Toggling `groupable` at runtime must flip `column.canGroupBy` so the Group/Ungroup affordance
+// appears and disappears accordingly.
+export const GroupableRuntimeToggleHarness = ({ initialGroupable = false }: { initialGroupable?: boolean }) => {
+  const [groupable, setGroupable] = useState(initialGroupable);
+  const columns = useMemo<AnalyticalTableColumnDefinition[]>(
+    () => [
+      { Header: 'Name', accessor: 'name', width: 200 },
+      { Header: 'Status', accessor: 'status', width: 200 },
+    ],
+    [],
+  );
+  return (
+    <>
+      <button data-testid="toggle-groupable" onClick={() => setGroupable((prev) => !prev)}>
+        toggle
+      </button>
+      <AnalyticalTable data={toggleData} columns={columns} groupable={groupable} visibleRows={10} />
+    </>
+  );
+};
diff --git a/packages/main/src/components/AnalyticalTable/test/AnalyticalTableGrouping.spec.tsx b/packages/main/src/components/AnalyticalTable/test/AnalyticalTableGrouping.spec.tsx
index 0ba592830d7..71a414c89fc 100644
--- a/packages/main/src/components/AnalyticalTable/test/AnalyticalTableGrouping.spec.tsx
+++ b/packages/main/src/components/AnalyticalTable/test/AnalyticalTableGrouping.spec.tsx
@@ -1,7 +1,13 @@
+import type { Page } from '@playwright/test';
 import { expect, test } from '../../../../../../playwright/fixtures/gallery-fixtures.js';
-import type { GroupingAggregationHarness } from './AnalyticalTableGrouping.gallery.js';
+import type { GroupableRuntimeToggleHarness, GroupingAggregationHarness } from './AnalyticalTableGrouping.gallery.js';
 
 const STORY = 'AnalyticalTableGrouping/GroupingAggregationHarness';
+const TOGGLE_STORY = 'AnalyticalTableGrouping/GroupableRuntimeToggleHarness';
+
+const listItem = (page: Page, text: string) => page.locator(`[ui5-li][text="${text}"]`);
+const popover = (page: Page) => page.locator('[data-component-name="ATHeaderPopover"]');
+const openHeaderPopover = (page: Page, text: string) => page.getByText(text, { exact: true }).click();
 
 test.describe('AnalyticalTable', () => {
   test('grouped column is aggregated on ancestor group rows', async ({ mount, page }) => {
@@ -21,4 +27,25 @@ test.describe('AnalyticalTable', () => {
     await expect(enUs).toHaveAttribute('data-agg-status', 'finished');
     await expect(enUs).toHaveText('all finished');
   });
+
+  test('runtime groupable toggle', async ({ mount, page }) => {
+    await mount<typeof GroupableRuntimeToggleHarness>(TOGGLE_STORY, { initialGroupable: false });
+
+    // Grouping is the only header option, so while disabled the header has no popover at all.
+    await openHeaderPopover(page, 'Status');
+    await expect(popover(page)).toHaveCount(0);
+
+    // Enable at runtime → the popover with the Group item appears, and grouping applies.
+    await page.getByTestId('toggle-groupable').click();
+    await openHeaderPopover(page, 'Status');
+    await expect(listItem(page, 'Group')).toBeVisible();
+    await listItem(page, 'Group').click();
+    await expect(page.getByText('active (3)', { exact: true })).toBeVisible();
+    await expect(page.getByText('inactive (2)', { exact: true })).toBeVisible();
+
+    // Disable at runtime → with no other option left, the header popover is gone again.
+    await page.getByTestId('toggle-groupable').click();
+    await openHeaderPopover(page, 'Status');
+    await expect(popover(page)).toHaveCount(0);
+  });
 });