diff --git a/livekit-rtc/livekit/rtc/event_emitter.py b/livekit-rtc/livekit/rtc/event_emitter.py
index 38f54901..65a14990 100644
--- a/livekit-rtc/livekit/rtc/event_emitter.py
+++ b/livekit-rtc/livekit/rtc/event_emitter.py
@@ -120,6 +120,11 @@ def once_callback(*args: Any, **kwargs: Any) -> None:
                 self.off(event, once_callback)
                 callback(*args, **kwargs)
 
+            # `inspect.signature` follows `__wrapped__`, so `emit` trims the arguments to
+            # what `callback` accepts. `_once_of` is set only on these wrappers, so `off`
+            # can match them to `callback` without touching other decorated listeners.
+            once_callback.__wrapped__ = callback  # type: ignore[attr-defined]
+            once_callback._once_of = callback  # type: ignore[attr-defined]
             return self.on(event, once_callback)
         else:
 
@@ -212,4 +217,8 @@ def greet(name):
             ```
         """
         if event in self._events:
-            self._events[event].pop(callback, None)
+            handlers = self._events[event]
+            handlers.pop(callback, None)
+            # a callback registered with `once` is stored wrapped, possibly more than once
+            for registered in [h for h in handlers if getattr(h, "_once_of", None) == callback]:
+                del handlers[registered]
diff --git a/tests/rtc/test_emitter.py b/tests/rtc/test_emitter.py
index 4f67f76a..ee0582fb 100644
--- a/tests/rtc/test_emitter.py
+++ b/tests/rtc/test_emitter.py
@@ -287,3 +287,108 @@ def handler() -> None:
     emitter.on("event", handler)
     emitter.emit("event")
     assert calls == ["x"]
+
+
+def test_off_removes_a_once_callback() -> None:
+    emitter = EventEmitter[str]()
+    calls: list[bool] = []
+
+    def on_event() -> None:
+        calls.append(True)
+
+    emitter.once("event", on_event)
+    emitter.off("event", on_event)
+    emitter.emit("event")
+
+    assert calls == []
+
+
+def test_off_removes_a_once_bound_method() -> None:
+    class Handler:
+        def __init__(self) -> None:
+            self.calls = 0
+
+        def on_event(self) -> None:
+            self.calls += 1
+
+    emitter = EventEmitter[str]()
+    handler = Handler()
+
+    emitter.once("event", handler.on_event)
+    emitter.off("event", handler.on_event)
+    emitter.emit("event")
+
+    assert handler.calls == 0
+
+
+def test_once_passes_only_the_arguments_the_callback_accepts() -> None:
+    emitter = EventEmitter[str]()
+    calls: list[tuple[Any, ...]] = []
+
+    def on_event(first: Any, second: Any) -> None:
+        calls.append((first, second))
+
+    emitter.once("event", on_event)
+    emitter.emit("event", 1, 2, 3, 4)  # only 2 arguments will be passed
+    emitter.emit("event", 1, 2, 3, 4)
+
+    assert calls == [(1, 2)]
+
+
+def test_once_with_no_parameters_ignores_emitted_arguments() -> None:
+    emitter = EventEmitter[str]()
+    calls: list[bool] = []
+
+    @emitter.once("event")
+    def on_event() -> None:
+        calls.append(True)
+
+    emitter.emit("event", 1, 2)
+
+    assert calls == [True]
+
+
+def test_once_passes_all_arguments_to_a_varargs_callback() -> None:
+    emitter = EventEmitter[str]()
+    calls: list[tuple[Any, ...]] = []
+
+    @emitter.once("event")
+    def on_event(*args: Any) -> None:
+        calls.append(args)
+
+    emitter.emit("event", 1, 2, 3)
+
+    assert calls == [(1, 2, 3)]
+
+
+def test_off_keeps_a_wraps_decorated_listener_of_another_callback() -> None:
+    emitter = EventEmitter[str]()
+    calls: list[bool] = []
+
+    def original() -> None:
+        pass
+
+    @functools.wraps(original)
+    def listener() -> None:
+        calls.append(True)
+
+    emitter.on("event", listener)
+    emitter.off("event", original)
+    emitter.emit("event")
+
+    assert calls == [True]
+
+
+def test_off_removes_every_once_registration_of_a_callback() -> None:
+    emitter = EventEmitter[str]()
+    calls: list[bool] = []
+
+    def handler() -> None:
+        calls.append(True)
+
+    emitter.once("event", handler)
+    emitter.once("event", handler)
+    emitter.off("event", handler)
+    emitter.emit("event")
+
+    assert calls == []