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Expand Up @@ -22,7 +22,7 @@ In this exercise, you will familiarize yourself with Piximi’s main functionali

#### **Context of the sample experiment**

In this experiment, researchers imaged fixed U2OS osteosarcoma (bone cancer) cells expressing a FOXO1A-GFP fusion protein and stained DAPI to label the nuclei. FOXO1 is a transcription factor that plays a key role in regulating gluconeogenesis and glycogenolysis through insulin signaling. FOXO1A dynamically shuttles between the cytoplasm and nucleus in response to various stimuli. Wortmannin, a PI3K inhibitor, can block nuclear export, resulting in the accumulation of FOXO1A in the nucleus.
In this experiment, researchers imaged fixed U2OS osteosarcoma (bone cancer) cells expressing a FOXO1A-GFP fusion protein and stained DAPI to label the nuclei. FOXO1A is a transcription factor that plays a key role in regulating gluconeogenesis and glycogenolysis through insulin signaling. FOXO1A dynamically shuttles between the cytoplasm and nucleus in response to various stimuli. Wortmannin, a PI3K inhibitor, can block nuclear export, resulting in the accumulation of FOXO1A in the nucleus.

<img class="content-img fig-center fig-single" width=300 src=../../img/translocation-tutorial/f0x01a.png>

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